Construction of LAMP detection method for poppy codeine in seasoning powders

OBJECTIVE: To establish the detection method for poppy codeine in seasoning power based on the loop-mediated isothermal amplification of DNA( LAMP). METHODS: Establishment of the LAMP reaction system and detection of 8 commercially seasoning powders by the specificity test, optimum temperature test and sensitivity test of DNA samples of 12 poppies seeds, 11 spices leaves or seeds and 1 corn poppy seeds based on the specific genes sequence of codeine. RESULTS: The DNA samples of the 12 poppies and the corn poppy showed the specific reaction, the optimum temperature was 66 ℃ and the minimum detectable concentration was 9 pg/μL, and the sensitivity was 100 times larger than that of PCR. There was codeine in one of the 8 commercially seasoning powders. CONCLUSION: The LAMP method applies to detect the poppy in seasoning powders with its high sensitivity, strong specificity, easy operation.