Genetic polymorphism of HPA1-17 alloantigen system in the Achang and Jingpo populations population in Yunnan.

BACKGROUND Human platelet alloantigen (HPA) is part of the platelet membrane structure, named HPA1- 17 system. This study aimed to investigate platelet alloimmunity in the Achang and Jingpo population, ethnic minority specific, to establish a typed platelet donor data bank in Yunnan's ethnic minority areas. METHODS In this study, samples from 139 unrelated healthy cases from the Achang population, 148 cases of the Jingpo population, and 150 healthy cases from Yunnan's Han population were collected as a control. PCR-sequence specific primers (PCR-SSP) methods were respectively adopted to genotype HPA-1-17. The frequency of genes and genotypes was calculated separately, and the frequency distribution of alleles in the Achang population was compared with that of the Han population. RESULTS Monomorphic HPA-7-14 and HPA-16 and 17 were found in the samples from the Han population, while HPA-b was not found in any of these samples. In HPA-1, 2, 4, 5, and 6, aa homozygosity was predominant. Monomorphic HPA-7, HPA-9-14 and HPA-16 were found in the samples from the Achang population, while HPA-b was not found. In HPA-1, 2, 4, 5, 6, 8, and 17, aa homozygosity was predominant. The genotype results of the HPA-4, 7, HPA9-12, HPA14, 16-17 antigen systems in the Jingpo population were all aa, while the HPA-b was not detected. In HPA-1, 2, 5, 6, 8 and 13, aa homozygosity was predominant. HPA-3, 15 showed the greatest heterozygosity among the three populations. The frequency of HPA-1a in the Achang population in Yunnan was significantly different from that in the Han population (P<0.05). There was no difference in the HPA system of the Jingpo population, but the HPA-2a system was different between the Achang and Jingpo populations (P<0.05). CONCLUSIONS In Yunnan, the distribution of the allele polymorphism of HPA-1-17 in the Achang and Jingpo population is similarly distributed to that in the Han population and exhibits their own characteristic. Therefore, a database of platelet donor typing of the ethnic group should be established.