Oncornavirus-like particles in HeLa cells. II. Immunological characterization of the virus
1974
An oncornavirus-like agent has been described in HeLa cells of human origin (Gelderblom et al., 1974). The investigation of its protein constituents revealed a pattern similar to but different from that of known oncornaviruses. Six polypeptides with molecular weights (mw) between 12,000 d and 28,000 d, and two glycopolypeptides of about 60,000 d and 80,000 d mw were detected by SDS-polyacrylamide gel electrophoresis. No serological cross-reaction was found between the HeLa virus and B- and C-type oncornaviruses. Essentially, none of two interspecies-specific antigenic determinants described in mammalian C-type viruses has been detected in the HeLa virus. However a distinct serological relationship was found between the HeLa virus and the Mason-Pfizer monkey virus in that these viruses share at least two antigens in the mw range of 16,000 d and 28,000 d, respectively. These results confirm the electron microscopic data described before (Gelderblom et al., 1974) and extend the evidence for a new group of oncornavirus-like particles.
Particules du Type Oncornavirus dans les Cellules HeLa. II. Caracterisation Immunologique du Virus
Un agent du type oncornavirus a ete observe dans des cellules HeLa d'origine humaine (Gelderblom et al., 1974). L'etude de ses composants proteiques a mis en evidence une structure analogue a celle des oncornavirus connus, mais pourtant differente. Six polypeptides d'un poids moleculaire (pm) de 12000 a 28000 d, et deux glycopolypeptides d'un pm d'environ 60000 d et de 80000 d ont ete deceles par electrophorese en gel de polyacrylamide-SDS. Aucune reaction serologique croisee n'a ete constatee entre le virus HeLa et les oncornavirus des types B et C. et surtout, aucun des deux determinants de l'antigene inter-especes observes dans les virus de type C des mammiferes n'a ete decele dans le virus HeLa. Toutefois, un lien serologique distinct a ete decouvert entre le virus HeLa et le virus de singe Mason-Pfizer; en effet, ces virus ont au moins deux antigenes communs d'un pm d'environ 16000 et 28000 d, respectivement. Ces resultats confirment les donnees recueillies au microscope electronique et deja signalees (Gelderblom et al., 1974) et apportent une nouvelle preuve de l'existence d'un groupe de particules du type oncornavirus jusqu'ici inconnu.
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