Hodgkin-Huxley parameters of the sodium channels in human myoballs

Myoballs were cultured from biopsies of adult human skeletal muscle without the use of antimitotic drugs. The sodium currents flowing during stepwise depolarization of the myoball membrane were investigated with the whole-cell recording technique. The temperature range covered 10–37°C. Two types of sodium channel were distinguished by their different sensitivity to tetrodotoxin (TTX). The channel with normal TTX sensitivity seemed identical with the sodium channel in adult muscle, the channel with less TTX sensitivity seemed identical with the juvenile channel found in developing and in denervated muscle. The activation and inactivation parameters of both channel types were quantitatively determined. The activation parameters of the two channel types were identical, but in comparison to theh∞-curve of the adult sodium channels theh∞-curve of the juvenile channels was positioned at more negative potentials, had a less steep slope, and when the temperature was decreased, its point of inflection shifted more in negative direction.