A Customizable Flow Injection System for Automated, High Throughput and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements

To better understand disease conditions and environmental perturbations, multi-omic studies combining proteomic, lipidomic, and metabolomic analyses are vastly increasing in popularity. In a multi-omic study, a single sample is typically extracted in multiple ways and various analyses are performed using different instruments. Thus, one sample becomes many measurements, making high throughput and reproducible evaluations a necessity. One way to address the numerous samples and varying instrumental conditions is to utilize a flow injection analysis (FIA) system for rapid sample injection. While some FIA systems have been created to address these challenges, many have limitations such as high consumable costs, low pressure capabilities, limited pressure monitoring and fixed flow rates. To address these limitations, we created an automated, customizable FIA system capable of operating at a range of flow rates (~50 nL/min to 500 µL/min) to accommodate both low- and high-flow instrument sources. This system al...