Diagnosis of multiple myeloma using enzyme immunoassay for detection of antibodies to N-acetyl glucosamine on microparticles

AbstractImmunoassay on polymeric particles with covalently bound N-acetyl glucosamine was applied for detection of appropriate antibodies. Antibodies to N-acetyl glucosamine were detected in sera of patients with multiple myeloma. The assay was conducted on polymeric microparticles by enzyme immunoassay. The sensitivities of the assays on microparticles and on glass microtubes were compared. It was shown that the level of antibodies to N-acetyl glucosamine correlated with the number of myeloma cells. The comparison of assays on microparticles and glass tubes demonstrate the advantage of microparticle technology.