Cultivo de Vibrio cholerae 638 en condiciones de estrés salino

Cholera is a disease caused by the bacterium Vibrio cholerae and is characterized by abundant watery diarrhea, this disease has been a serious health problem since the first pandemic record in 1817. In 2009, the World Health Organization (WHO) reported more than 220,000 cholera cases and nearly 5,000 deaths in 45 countries. However, these reports are believed to be underestimated because in many countries where cholera is endemic, cases are not reported.1 Cholera is endemic in South Asia, in parts of Africa and Latin America, where seasonal outbreaks occur.2 During the past 20 years, the highest reported incidence shifted from America to Africa. The latter represented 98% of the reported cases of cholera and 99% of deaths related to this disease during 2009.1 The attenuated strain 638 of Vibrio cholerae (VC 638) comes from a second generation of genetically modified strains, derived from the virulent strain C7258 V. cholerae 01 El Tor Ogawa, isolated during the Peruvian epidemic in 1991.3,5 This strain was selected for develop a vaccine candidate, as it is immunogenic and not very reactogenic.6