饲料n-6/n-3多不饱和脂肪酸比例变化对小鼠血浆瘦素表达的影响

Objective To explore the effects of dietary ratio changes of n-6/n-3 polyunsaturated fatty acids (PUFAs) on expression of plasma leptin and soluble leptin receptor (sOB-R) in mice. Methods Fifty of 3-4 week-old C57BL/6J female mice, which were randomly divided into 5 groups (10 cases in each group), were fed 4 diets with different ratios of n-6/n-3 PUFAs (20:1, 10:1, 5:1 and 1:1) for 3 months, and n-3 PUFAs deficient diet as controls. The amount of fat in each diet was 6% (weight percentage), and n-6 and n-3 PUFAs were originated from sunflower oil and fish oil, respectively. Plasma leptin and sOB-R concentrations were measured by enzy-me-linked immunosorbent assay (ELISA). SPSS 11.5 software was used to analyze the data. Results There were no significant difference among the weight of 3 groups after different diets of n-6/n-3 PUFAs fed for 3 months. Compared with n-3 PUFA deficient diet, plasma leptin and sOB-R concentrations in mice fed with n-6/n-3 PUFAs (1:1) diet were significantly reduced (F=73.31 P=0; F=7.20 P=0.03), whereas those in mice fed with n-6/n-3 PUFAs (20:1), (10:1) and(5:1) did not change significantly. Conclusions Changes in dietary ratio of n-6/n-3 PUFAs affect the expression of leptin and sOB-R, and higher amount of n-3 PUFAs is needed to reach the inhibitory effects of leptin and sOB-R expression.