Analysis of pathogens and antibiotic resistance in nosocomial urinary tract infections.

Objective To study the distribution and antibiotic resistance of pathogens in nosocomial urinary tract infection and to provide evidence for administration of antibiotics.Methods A total of 156 pathogen strains were isolated from urine samples of patients with nosocomial urinary tract infection.Bacteria were identified and drug sensitivity analysis was conducted.Production of extended-spectrum β-lactamase(ESBLs)was tested for isolates of Escherichia coli and Klebsiella pneumoniae.Results The major pathogens of nosocomial urinary tract infection were Gram-negative(G-) bacteria(n=72,46.15%),followed by fungi(n=55,35.26%) and Gram-positive(G+)bacteria(n=29,18.59%).The most common strains isolated were Escherichia coli,Candida tropicalis,Candida albicans,Enterococcus faecalis and Klebsiella pneumoniae in sequence.The proportions of ESBLS production were 57.50% and 73.33% for Escherichia coli and Klebsiella pneumoniae respectively.Pathogens had drug resistance to multiple common antibiotics.The sensitivity rate of G-bacteria was 100.0% to polymyxin B,and was higher than 80.0% to cefoperazone/ sulbactam,piperacillin/tazobactam,furantoin,carbapenems,ampicillin and ciprofloxacin respectively.The sensitivity rate of G+ bacteria was 100.0% to vancomycin and oxazolidinone antibiotics respectively,and was higher than 80.0% to penicillin G.Conclusion Drug resistant strain of pathogens played an important role in urinary tract infection.The results suggested that drug sensitivity test was critical in antibiotics selection for the treatment.