Enhancing the establishment efficiency of hybridoma cells: use of irradiated human diploid fibroblast feeder layers

Abstract Eight different cell feeder layers and 2 conditioned media were compared for their ability to increase the establishment efficiency of newly formed hybridoma cells. Enhancement was noted particularly with all 3 irradiated human lung diploid fibroblasts which were tested. Hybridomas established in the presence of the feeder layers were found to be stable upon propagation even in the absence of the feeder cells. MRC-5 diploid fibroblasts are suggested as the hybridoma feeder layer cell of choice due to this cell's ease of culture and handling.