The Expression of VEGF in the Low Dose Ultrashortwave Repairs Rats Sciatic Nerve Lesions by Using Acellular Allogenic Nerve Scaffold Implanted with Bone Mesenchymal Stem Cells

Objective To observe the expression of vascular endothelial growth factor(VEGF)in the low dose ultrashortwave repairs rats sciatic nerve lesions by using acellular allogenic nerve scaffold implanted with BMSC.Methods BMSC were injected into the prepared acellular nerve scaffold and maintained in DMEM complex culture medium for 72 h until the experiments were performed,then the graft sutured into defective sciatic nerve.Adult male Wistar rats(200～250 g)were divided into DMEM control group,BMSC-treated group and BMSC-treated ultrashortwave(USW)therapy group.USW groups began to therapy with the low dose ultrashortwave at 2 days after surgery.At 2,4,8 and 12 weeks after surgery,animals were sacrificed and took out of the regenerated nerves to detect the expression of VEGF and S100.Results The VEGF expression can be detected in regenerated nerves.The results displayed the positive expression of VEGF increased to peak 4 weeks after surgery,then decreasd gradually,meanwhile its expression were highest at same stage in USW group compaired with BMSC group and DMEM group.Conclusion Ultrashortwave therapy were able to promote or accelerate BMSC differentiate into Schwann cells or to promote Schwann cells'proliferation and migration.The high expression of VEGF at early stage of nerve repairing concluded that the low dose ultrashortwave was able to provide more blood supply for nerve regeneration.