Identification of 17–18 kDa zona pellucida binding proteins from boar spermatozoa

Abstract Zona pellucida (ZP) binding proteins from boar spermatozoa were compared with antigens recognized by ACR.2 and ACR.3 monoclonal antibodies. The ZP binding proteins of 55, 53,45 and 38 kDa are identical with various forms of boar acrosin immunologically detected by ACR.2 antibody. The ZP binding proteins of 17 and 18 kDa are recognized by ACR.3 antibody. The N-tenninal amino acid sequence of the 17 kDa protein revealed that it is not derived from an acrosin molecule.