Development and application of fluorescent, green light-activatable caged compound
2011
Caged compound is one of the most powerful tools for spatiotemporal control of biomolecules in cells, which can be
activated by irradiation of light. However, ultra violet light, which is required for activation of caged compounds, can
damage cells and has poor permeability into tissues. In addition, invisibility of caged compounds makes it difficult to tell
distribution of released small molecules. At the conference, we will describe the development of novel caging group and
new caged compounds which are fluorescently visible and efficiently activatable with green light. We have found that
boron dipyrromethene (BODIPY), known as a widely used fluorophore, is a potential caging group for phenol, carboxyl
acid and amine, which can be photolized with irradiation of green light at around 500 nm wavelength. Based on the
novel photo-reaction of 4-phenoxy BODIPY derivatives, we have developed caged histamine and applied it to HeLa
cells. Photo-irradiation to cells in the presence of caged histamine induced transient increase of calcium ion in cytosol,
which was specifically inhibited with pyrilamine, a H1 blocker. Also, we showed that BODIPY-caged compound can be
utilized in vivo with tissue-permeable 500 nm green light.
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