prostaglandin J 2 on Amnion-Derived WISH Epithelial Cells: Differential Roles of Peroxisome Proliferator-Activated Receptors and and Nuclear Factor B

Deoxy 12,14 -prostaglandin J2 (15d-PGJ2), an activator of per- oxisome proliferator-activated receptor (PPAR)- and -, is a pro- stanoid metabolite with anti-inflammatory actions. In intrauterine tissues, proinflammatory cytokines and prostaglandins have been identified as playing key roles in the maintenance of pregnancy and the onset of labor. We investigated and compared the early (3 h) effects of 15d-PGJ2 with rosiglitazone (PPAR- ligand) and 2-methyl-4-((4-methyl-2-(4-trifluoromethylphenyl)-1,3-thiazol-5- yl)-methylsulfanyl)phenoxy-acetic acid (GW501516) (PPAR- li- gand) on interleukin (IL)-1-induced prostaglandin and cytokine production by amnion-derived WISH cells. We show that 15d- PGJ2 exerts differential effects depending on concentration. At low concentrations (0.1 M), 15d-PGJ2 inhibited IL-1- stimulated prostaglandin E2 (PGE2) but not cytokine (IL-6/IL-8) production or cyclooxygenase-2 (COX-2) expression. This effect was attenuated by a PPAR- inhibitor (2-chloro-5-nitro-N-phenyl- benzamide (GW9662)), by transfection with a dominant-negative