HPLC (High Performance Liquid Chromatography) quantitative method for cephaeline hydrochloride and ipecine hydrochloride in ipecacuanha medicinal material and preparation of ipecacuanha medicinal material

The invention relates to a HPLC (High Performance Liquid Chromatography) quantitative method for cephaeline hydrochloride and ipecine hydrochloride in an ipecacuanha medicinal material and a preparation of ipecacuanha medicinal material. The method is characterized by comprising the following steps: firstly, performing common isocratic elution according to PHLC method, and taking acetonitrile-carbinol-0.1% of phosphoric acid at a volume ratio of (8-9.5):(3-5):(86-88) as a flowing phase, wherein a detecting wavelength is 205nm; and simultaneously measuring the contents of the cephaeline hydrochloride and ipecine hydrochloride in the ipecacuanha medicinal material and a fluid extract, extract and tincture thereof so as to end the history of the measurement of total alkaloids of the ipecacuanha alkaloids in an acid base titration form according to the standard in multiple countries: after performing column chromatography separation for 4 times, respectively measuring the cephaeline hydrochloride and ipecine hydrochloride through delta A at 283nm and 350nm by adopting differential spectrophotometry. According to the method, only acidic aqueous carbinol or aqueous carbinol is used; after fine powder of the medicinal material is ultrasonically extracted or each preparation is diluted, a certain amount of subsequent filtrate is absorbed; and an alumina column is used for removing impurities so as to measure. The method is quick, convenient, accurate and capable of reappearing.