Analytical method for measuring the k-caseinolytic enzyme activity.

Analytical method for measuring the activity lytic enzyme of interest in the cheese industry and in biological research. It is based on measuring the amount of The method comprises the following steps: 1) Preparation of substrate, FTC 2) Incubation of an enzyme sample in the presence of FTC 3) The reaction is stopped by addition of trichloroacetic (TCA). 4) The emitted fluorescence is obtained by measuring an aliquot of the reaction product using a fluorimeter. 5) Activity values are extracted from the equation of a straight pattern relating the fluorescence to the amount of