[Analysis of cholesterol ester transfer protein gene Taq IB and -629 C/A polymorphisms in patients with endogenous hypertriglyceridemia in Chinese population].

Objective To investigate the variations of cholesterol ester transfer protein(CETP)gene and its relation to endogenous hypertriglyceridemia(HTG)in Chinese population.Methods One hundred and thirty-five endogenous hypertriglyceridemics and 214 healthy subjects from a population of Chinese Han nationality in Chengdu area were studied using restriction fragment length polymorphism(RFLPs)amplified by polymerase chain reaction(PCR).The polymorphic sites studied included Taq IB and-629 C/A polymorphism in CETP gene.Results The frequencies of B_2 allele at Taq IB site in normal group and HTG group were 0.418 and 0.382,respectively.The frequencies of A allele at-629 C/A site in the two groups were 0.479 and 0.489,respectively.No significant difference between normal control and HTG groups were found in both allele frequency of the two polymorphism.Linkage disequilibrium was observed between Taq IB and-629 C/A polymorphic sites(D'=0.881).In the normal control group,subjects with genotype B_2B_2 of Taq IB site had a higher serum mean concentration of HDL-C and lower LDL-C when compared with that of genotype B_1B_1 and B_1B_2,respectively(both P0.05),while those with genotype CC of-629 C/A site had a lower LDL-C level and higher Apo AⅡ level when compared with that of genotype AC(P0.01 and P0.05,respectively).The changes of the lipid and lipoprotein levels were only observed in normal male subjects when male and female groups were further separated.No significant changes of lipid and lipoprotein levels were observed in both polymorphism in HTG group.Combined genotype analysis of the two sites,subjects with genotype B_2B_2CC in normal controls had higher HDL-C levels but lower serum triglyceride(TG)when compared with B_1B_1CC.Conclusion These results suggest that Taq IB and-629 C/A polymorphisms in CETP gene are associated with healthy control subjects to some extent in Chinese population,but not with endogenous hypertriglyleridemia in the population group.