Anti-radical mechanism of the antihypertensive effect of Wakouba an extract from palm oil

Oxidative stress is a pathology responsible for several diseases. It is characterized by a relative increase of the reactive species of the oxygen (ERO) and can decrease the bioavailability of NO. The oxidative stress, having for consequence the appearance of several often irreversible damage at the level of cells, constitutes one of the important factors at the origin of the arterial high blood pressure. It so appears as a potential factor of arisen several diseases of which arterial blood pressure. Therefore, an efficacy treatment of the arterial high blood pressure could be also an antioxidant. From this perspective, this study allowed us to establish the anti-radical mechanism of Wakouba an antihypertensive product used in the traditional environment. The extracts of Wakouba are obtained after maceration in the methanol 96 %, during 48 hours in the ambient temperature (25°C), followed by double filtration on cotton then on paper tram driver 3 mm and evaporated with the rotavapor. So, the powder obtained allowed us to realize our antioxidant tests. Various doses of Wakouba and Tenordate the antihypertensive product used as reference were administered to rabbits made spontaneously hypertense has the hanging adrenalin for 7 days. The animals were then sacrificed to take the heart, the liver, the kidney and the aorta for the dosage of superoxide dismutase and the catalase. Furthermore, the inhibition of the lipid peroxidation was made by the method in the ferric thiocyanate and the method of the TBARS. It emerges from this study that the treatment by various doses of Wakouba and Tenordate in the hypertense animals decreases significantly until the normalization of the activity of the SOD and the catalase beforehand raised in the aorta, the heart, the liver and the loins. On other hand, Wakouba inhibits the lipid peroxidation in 78,3% as well as the Vitamin C reference molecule which exercises 81,00%. This consolidates us for its use as antihypertensive substance in the traditional environment.