茄紅素、α-胡蘿蔔素與β-胡蘿蔔素高效液相層析法之建立

The aim of this study is to elucidate the effect of raito of acetonitrile and methanol on separating lycopene, α-carotene and β-carotene by HPLC with RP-C18 column. Results showed that the three carotenoids were separated by isocratic mobile phase, the higher methanol ratio of isocratic, the less analysis time and better separation effect of lycopene. In constrast, higer methanol ratio led to the lower separation effect of α-carotene and β-carotene. Eluention with acetonitrile and methanol binary gradient mobile phase showed a better consistency and recovery in this research.