Distribution and factors associated with salivary secretory leukocyte protease inhibitor concentrations

Objectives This cross-sectional study examined the distribution and correlates of salivary secretory leukocyte protease inhibitor (SLPI) concentrations within a multinational cohort of men. Methods Extracellular SLPI was measured in oral gargle cell supernatants of 378 men from three countries using an ELISA-based assay. Risk factor data were collected by a questionnaire. Factors associated with SLPI were assessed using linear and logistic regression for continuous and categorical SLPI, respectively. Results Among men ages 18-73 years, the median SLPI concentration was 492.0 ng/mL (range: 2.3–1919.9). In multivariable modeling, men in Brazil and younger men (18–30 years) were more likely to have higher levels of SLPI, (adjusted odds ratio (aOR) 3.84; 95% confidence interval (CI): 1.94-7.59, and aOR 3.84; 95% CI: 1.98-7.43, respectively). Men with a self-reported STD diagnosis in the past six months were more likely to have higher SLPI levels (aOR 2.98; 95%CI: 1.1-7.83) and men reporting bleeding/swollen gums were less likely to have higher SLPI, (aOR 0.34; 95%CI: 0.15-0.79). Similar results were observed for linear regression models. Conclusions SLPI concentrations varied significantly by country and decreased with increasing age. The interaction between SLPI, modifiable factors, and oral infections that influence cancer risk warrants further investigation. This article is protected by copyright. All rights reserved.