Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse.

Abstract To improve the enzymatic digestibility of sweet sorghum bagasse and bioethanol production, five pretreatment methods have been investigated and compared, including (1) dilute NaOH solution autoclaving pretreatment, (2) high concentration NaOH solution immersing pretreatment, (3) dilute NaOH solution autoclaving and H 2 O 2 immersing pretreatment, (4) alkaline peroxide pretreatment and (5) autoclaving pretreatment. Among them, the best result was obtained when sweet sorghum bagasse was dilute NaOH solution autoclaving and H 2 O 2 immersing pretreatment. The highest cellulose hydrolysis yield, total sugar yield and ethanol concentration were 74.29%, 90.94 g sugar/100 g dry matter and 6.12 g/L, respectively, which were 5.88, 9.54 and 19.13 times higher than the control. Moreover, the FTIR and SEM analysis illustrated significant molecule and surface structure changes of the sweet sorghum bagasse after pretreatments.