Detection of some resistence gene of Salmonella isolated from turkey

The aim of this study was to determine the serotyping and antimicrobial resistance of isolated Salmonella from the appearently healthy turkey. A total of 150 (cloacal samples) from living apparently healthy turkeys samples were screened bacteriologically for the occurrence of Salmonella. A total of 6/150 (4%) isolates of Salmonella was recovered. Serotyping revealed two different serotype, S.Enterica subspp Salamae (33.33%) and S.Kentucky.(66.67%). The isolated Salmonella were highly resistant to ampicillin, cefaclor, cefotaxime, ceftazidime, amoxicillin/clavulanic acid(100%) followed by chloramphenicol and ciprofloxacin (83.3%) then gentamycin (66.67%) and azithromycin (33.3%). All isolates showed high sensitivity for imipenem. All strains are multidrug-resistance (MDR). Polymerase chain reaction (PCR) was applied on Salmonella isolates to detect resistance gene. Antibacterial resistance genes blaTEM, blaOXA, floR, aadB and qnrA were detected in (100%), (0%), (100%), (100%) and (0%) of tested Salmonella. A combination of genotypic and phenotypic markers can be useful in studying genetic variation among salmonellae populations in turkey production and delineating possible transmission pathways. Conclusion: This study demonstrates that appearently healthy turkeys could be a reservoir for Salmonella resistant to multiple antimicrobials and poses a serious public health threat.