Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b.

Abstract Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water–methanol extraction followed by a chromatography on a C 18 -support. The analysis with LC–MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C 15 , fengycin/plipastatin A-C 16 together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b.