Biogenic Synthesis of AGNP's Using Pomelo Fruit - Characterization and Antimicrobial Activity against Gram +Ve and Gram -Ve Bacteria

In the last decade synthesis of silver nanoparticles has been a subject of a lot of study and numerous methods are being developed for synthesis of nanoparticles of various shapes and sizes. Various biosynthetic strategies are being developed to employ biological sources such as fungi, bacteria, viruses, actinomycetes, algae and plant extracts to catalyze specific reactions. The use of citrus fruit extract to synthesize silver nanoparticles is an exciting possibility that relatively unexplored and under exploited. In the present study, the cost effective and ecofriendly technique of green synthesis of silver nanoparticles using Pomelo fruit (Citrus maxima) extract was demonstrated. The reduction process was simple, rapid and convenient to handle. Primary detection (color change of solution) indicated the formation of silver nanoparticles and was monitored by UV –Vis Absorption Spectroscopy. Characterization of silver nanoparticles was analyzed with X-ray Diffraction (XRD), Energy Dispersive Spectroscopy (EDS), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscope (TEM) and Particle Size Analysis (PSA). The antimicrobial activity of synthesized silver nanoparticles showed effective inhibitory activity against different multi-drug resistant human pathogens.