Ag-nanofilm antimicrobial system on the base of bacterial cellulose

There has been increased concern during the past few decades over the last-line antibiotic resistant organisms reported from many parts of the world. Although bacterial nanocellulose (BNC), product of Komagataeibacter bacteria strains, offer unique material characteristics, it does not exhibit antibacterial activity. In present study, we produced a coating which is relative to bacterial nano-cellulose (product of Gluconacetobacter sucrofermentans B-11267) loaded with N’,N’-hexane-1,6-diylbis[N-(4-chlorophenyl) (imidodicarbo-nimidic diamide)] and covered with thin silver films from 1 to 30 nm. For making thin layer of silver, magnetron sputtering (ETNA100- MT, NT-MDT) was used on BNC dried to a constant weight without heating of sample. The surface modification of the BNC was confirmed using scanning electron microscopy. Cytotoxicity tests of cellulose nanofibril-based structures on fibroblasts culture identified moderate cytotoxic effect. Significant antibacterial activity of the composite was achieved for Gram-negative as well as for Gram-positive bacteria.