Process for sequencing a polynucleotide mold

A method for sequencing by pairs of first and second regions of a ofDouble polynucleotide target strand, wherein said first and second regions are in the same polynucleotide double hebradiana, the method comprising: (a) providing a solid support having immobilized thereon a plurality of polynucleotides ofDouble template strand each formed from first and second complementary strands bonded solid mold alsoporte at their 5 'and multiple copies of one or more primers immobilized on the end of hybridizing 5'capaces 3 'end of the first template strand; (B) selectively removing the second template strands of the plurality of polynucleotides doblehebra mold to allow hybridization of the first mold strands with primers immobilized on the extremo5 '; (C) carrying out a first reading of sequencing to determine the sequence of a first polynucleotide Region from mold by sequencing by synthesis technique or by a technique desecuenciacion by ligation; (D) carrying out an extension reaction to extend one or more primers immobilized To copy to the first strand of the template to generate a second strand of the immobilized mold; (E) selectively removing the first template strands of the plurality of polynucleotides doblehebra mold to allow hybridization of a sequencing primer to the template strands generated in laetapa (d); (F) performing a second reading sequencing to determine the sequence of a second polynucleotide Region from mold, by a technique of sequencing by synthesis or by a technique desecuenciacion by ligation, which determine the sequences of the first and second regions delpolinucleotido sequencing target achieved by pairs of said first and second regions dichopolinucleotido double stranded target.