Enhanced Signal Generation by Target Amplification
1992
The polymerase chain reaction (PCR) represents the most common and widespread method for the direct amplification of specific sequences of nucleic acid target molecules. The basic reaction is comprised of three steps:
1.
Denaturation of the target DNA
2.
Annealing of sequence specific primers
3.
Template-specific elongation of these primers with a DNA polymerase and desoxynucleotides
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