Mycobacterium avium subspeciae paratuberculosis identification in milk by means of IS900 PCR.

Between the numerous emerging pathogens, Mycobacterium avium subsp. paratuberculosis (MAP) reports a primary interest in its detection for its possible correlation with Crohn’s Disease in man (5). Milk and its subproducts may be very important in transmitting Mycobacterium avium subsp. paratuberculosis. The potential sanitary risk is related to the germ capacity in surviving to routine pasteurization treatments (1). These study objectives were the determination and the optimization of DNA extraction and purification assays to the direct Mycobacterium avium subsp. paratuberculosis (MAP) detection in milk, by means of PCR. The obtained results with the applied method are based in the milk and its subproducts chain inspective and control actions, relating to the increasing paratuberculosis etiological agent presence and the potential zoonotic transmitting.