Monomer conversion, release and cytotoxicity analyses of experimental GDMA-P based adhesives

Abstract This study aimed to evaluate the effects of a photoinitiator system and acidic monomer (GDMA-P) concentrations on self-etch experimental dental adhesive degree of conversion (DC), monomer release and cytotoxicity. Three monomer blends containing different GDMA-P/HEMA/UDMA ratios were produced: 1/3/3; 2/3/2; and 3/3/1. CQ + EDMAB; BAPO or CQ + BAPO + EDMAB were used as photoinitiator systems. DFI was added as co-initiator, and a water/ethanol mixture was used as solvent. DC was evaluated by Fourier transform infrared spectroscopy (FTIR) (n=10). Monomer release was identified by ultra-high performance liquid chromatography (UHPLC) (n=6). The Alamar Blue assay was used to test NIH-3T3 fibroblast cell viability (n=8). A two-way ANOVA and Tukey post-hoc test were used for the data analyses (p