Free Paper Presentation : F-144 ; Oral Intake of Anti-hangover Substance Increases Metabolizing Capacity of Aldehyde Dehydrogenase in Rat Model: New Preventive and Therapeutic Potentials for Influenza Virus-Induced Lung Injury?

Background: Aldehyde dehydrogenase (ALDH) metabolizes acetaldehyde, the major cause of alcohol hangover symptoms. It also detoxifies cytotoxic reactive aldehydes. Influenza virus-induced oxidative stress promotes lipid peroxidation of cellular membrane, leading to the accumulation of reactive aldehydes that contribute to lung injuries. A variety of anti-hangover products are commercially available, however, almost none of them has been proven to show enhanced metabolizing capacity of ALDH in a live subject. We aimed to test a specific product of interest. Methods: A powder sample of anti-hangover (KISLip, Pico Entech, Korea) was examined by in vitro & in vivo experiments to measure the amount of NADH formation which is generated through catalytic conversion of acetaldehyde. In-vivo examination tested the ethanol and acetaldehyde level in blood of rats with oral infusion of substance before or after ethanol intake. Results: The activities of alcohol dehydrogenase & aldehyde dehydrogenase within the anti-hangover substance were 1.84 unit/g and 0.28 unit/g, respectively. The oxidation capacities of rats were dose-dependently increased after substance gavages. Particularly, the cases with oral intake of substance 220 mg/kg after 1hr of ethanol intake have shown more meaningful decreases in acetaldehyde level in blood. Conclusions: Oral intake of anti-hangover substance has significantly enhanced acetaldehyde-metabolizing capacity in rat model, potentially suggesting increased ALDH capacity within circulation and detoxifying ability of other reactive aldehydes.