Entwicklung und Charakterisierung eines Enzym-Immunoassays mit polyklonalen Antikörpern für Benzol, Toluol und Xylole (BTX)

An indirect competitive enzyme immunoassay for the detection of the volatile organic compounds benzene, toluene, and xylenes has been developed with polyclonal antibodies. The limit of determination for the equal amount of the five analytes in water was 210 μg/l with a center point value of 1.7 mg/l. The addition of 10% of dimethyl sulfoxide (DMSO) to the sample decreased the limit of determination to 80 μg/l and the center point value to 540 μg/l. The specificity of the polyclonal antibodies was investigated based on its cross-reactivity. The influence of increasing concentrations of organic solvents and humic acid on the sensitivity of the antibodies was studied. Water samples were analysed both with GC-FID and by an immunochemical method in order to evaluate the suitability of the assay for environmental analysis.