Preparation method for chickpea antibacterial peptides
2013
The present invention discloses a method for preparing chickpea antibacterial peptide, comprising: step 1, chickpea protein prepared by mixing flour with water 3 to 8% by weight of the suspension; Step 2, to adjust the protein powder chickpea pH of the suspension was 6.0 to 7.5, per gram of protein powder added 3000-4000U chickpea forming enzyme hydrolysis system; step 3, 1.0 ~ 1.5h enzymatic reaction system at a temperature of 45 ~ 50 ℃, the enzymolysis temperature was raised immediately after the completion of the reaction to enzyme inactivation ≥95 ℃ 10 ~ 15min, to obtain hydrolysates; step 4, the centrifugal separation of the hydrolyzate, the supernatant using an ultrafiltration membrane peptides entrapped to give molecular weight between the peptide segments system 2kDa-6kDa; peptides in a spray drying system for chickpea inhibitory peptides. Advantageous effects of the invention are: low cost, simple operation process, while allowing to improve the antibacterial peptide hydrolysis product yield.
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