Method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as fluorescent probe to detect copper ions

The present invention discloses a method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as a fluorescent probe to detect copper ions, and belongs to the technical field of optical analysis detection. The recognition effect of the 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound and various metal ions is investigated and studied experimentally, a good copper ion selective recognition effect of probe molecule ESN is found, due to the solubility of hydroxyl and water, the probe molecule can achieve the fluorescence identification of the copper ions in an aqueous solution, a better result can be achieved under physiological pH condition, the method is green, specific in copper ion selectivity and relatively high in sensitivity, and can play fluorescence quenching role in short reaction time, and the probe molecule can be successfully applied to the detection of the copper ions in biological living cells.