Influence of fluoride concentration on ameloblast activity in vitro culture

Objective To develop the primary culture technique of rat ameloblasts and observe the influence of different concentrations of sodium fluoride on ameloblast activity in vitro culture to furnish basis for the study of dental fluorosis formation.Methods Take 10 ~ 15 d old Wistar rat molar tooth germ tissue for primary culture.The enzymes digesting method was used to isolate and culture the ameloblasts.Then ameloblasts were exposed to different concentrations of NaF(0,0.4,0.8,1.6,3.2,6.4 mmol / L) for 24,48 and 72 h.CCK-8 was used to detect cell activity.Results The proliferation of ameloblasts was promoted by NaF at 0.4 and 0.8 mmol / L,and the effect was strengthened as time increased whereas it was inhibited by 1.6,3.2 and 6.4 mmol / L NaF.With the increase of NaF concentration and the time,the inhibitory action was also strengthened and became more obvious.Conclusions Fluoride concentration has a biphasic effect on ameloblast activity in vitro culture: at low doses,it promotes cell proliferation while at high doses it has negative effects.