Application of lactic acid bacteria vector pMG36e.

Plasmid pMG36e,based on protease gene transcription and translation signals from Lactococcus lactis cremoris,is a typical artificial constitutive expression vector as a main expression system in Lactococcus. It has a strong promoter and has been used for expression of heterologous protein such as enzyme in a variety of bacteria. In recent years,it was used to investigate the mechanism of bacteriocin as an intermediate vector,to construct lactic acid bacteria genetic strains and develop oral vaccine,even in gene therapy study. pMG36e has been used so widely in multi-disciplinary applications,it becomes one of the most important plasmid in the lactic acid bacteria genetic engineering. The basic plasmid composition,structure,and application of pMG36e in gene expression and food-grade vector construction were reviewed to provide reference for future research.