DETECTION OF ANTIMICROBIAL EFFECT OF EXTRACELLULAR PROTEIN PRODUCED BY PSYCHROPHILIC ACTINOMYCETES ISOLATED FROM MANALI ICE POINT

The present study reveals that the effect of extra cellular protein isolated from Psychrophilic Actinomcetes against clinical pathogens. Totally 13 Actinomycetes were isolated from glacier soil. Among them the genus Streptomycetes and Micromonospora were isolated highly. MM9 medium was used as fermentation medium and the antimicrobial peptide activity was performed by disc diffusion method. Out of 14 isolated actinomycetes  C2, I and J were found to be effectively produce antimicrobial Extracellular protein active against 3 test pathogens . The most important significant finding of this work is antimycobacterial activity of extracellular protein produced b y Streptomycetes (C2) and Micromonospora sp (J) were active against Mycobacterium tuberculosis. The zone of inhibition was 20 mm (C2) and 22mm (J) respectively. Among the C2, I and J isolates Micromonospora sp (J) is most effective towards all three test organism. The molecular weight of antimycobacterial protein was 39,000 Da (C2) and 3000 Da (J)