Expression of 45W-4B gene of Taenia solium in the silkworm

Expression system of BmNPV-Bm is high effective for producing recombinant protein approach to its nature structure by the correct post-transcript process and modification.45W-4B gene of Taenia solium was cloned into baculovirus transfer vector of pVL1393 and cotransfected into Bombyx mori nuclear polyhedrosis virus(BmNPV).The recombinant BmNPV was screened,and used to infect BmN cells and silkworm.4B protein of expression was detected in the cell supernatant and haemolyph by Western blot.Successful expression of 4B recombinant protein in BmNPV-Bm will be significant for study of the structure and function of 4B protein,and development of the effective recombinant vaccine against T.solium and Cysticercus cellulosae.