[Effect of Ras signal regulating Cox7a2 expression and its sub-cellular location in testicular interstitial cell].

Objective:To investigate the co-sub-cellular-location of Cox7a2 and Ras.Methods: Ras and its mutant plasmid were cloned by RT-PCR and sequence analysis.Cox7a2-pEYFP-N1,Ras-pEYFP-N1 and N17-Ras-pEYFP-N1 fluorescent protein vectors were constructed and transfected into TM3 cells.Results: Cox7a2 was located in the mitochondria,but its location was changed by the expression of Ras.When the dominat negative ras was expressed in the cells,the Cox7a2 located into the mitochondria again.Conclusion: Cox7a2 mediated testosterone production,which might be at least in part related with the Ras signaling pathway.Ras may be the regulating target and further investigation is needed to make it clear.