A modified method for rapid quantification of Chlamydia muridarum using Fluorospot

Abstract Although manual enumeration of Chlamydia inclusion forming units is the most widely accepted means of quantification in the field, it is both time consuming and subject to inherent investigator bias. We report here a rapid, i.e., minutes vs. hours, modified automated Fluorospot means of assessment that is linear ( • Concurrent enumeration of chlamydial IFU using automated and manual methods of counting on same tissue culture plate. • Rapid method of counting chlamydial IFU reducing time from hours to minutes.