Optimization of Agrobacterium-Mediated Soybean Transformation of Cotyledonary-Node

The efficiency of Agrobacterium-mediated soybean transformation of cotyledonary-node was affected by some factors including receptor genotype,germinating condition,shoot induction,elongation and rooting.10 soybean cultivars were chosen,Dongnong 50 in 10 tested cultivars was used as the transformed receptor species,and the system of cotyledonary-node transformation was established.We investigated the proper dosage of 6-BA in germination medium and rooting mode.The results showed that different concentration of 6-BA was added in germination medium and the rate of elongation was 2.51%~20.59% with 0 concentration of 6-BA in germination stage.Dipping the stem in 1 mg/L IBA for 1 minute and rooting in 1/2 MS medium shortened the rooting time and increased rooting rate by 85.25%.The transforming rate of the system could achieve 7.10% by PCR assay.