Цитокины и матриксные металлопротеиназы при патологии верхних отделов пищеварительного тракта у детей

The paper gives the data of examination in 310 children (mean age 11.1±4.7 years) with inflammatory and destructive lesions of the gastric and duodenal mucosa. All the patients underwent diagnostic esophagogastroduodenoscopy. The serum concentrations of interleukins (IL) (IL-6, IL-8, and IL-10), tumor necrosis factor-a (TNF-α), matrix metalloproteinases (MMP-2, MMP-9, their tissue inhibitor (TIMP-1), and endothelin-1 were simultaneously measured by a solid-phase enzyme-linked immunosorbent assay (ELISA). Endogenous nitric oxide (NO) production by a gastric mucosal homogenate was estimated by a spectrophotometric assay at a wavelength of 520 nm. Pronounced mucosal Helicobacter pylori (Hp) colonization-associated changes in cytokines and MMP were ascertained in inflammatory and ulcerative damages in the gastrointestinal mucosa. The cytokines and MMP were shown to be involved in the diagnostic algorithm to estimate the degree of erosive-ulcerative lesions in the upper gastrointestinal tract and to be used to determine new lines in the diagnosis and treatment of these forms of the diseases in children.