Anti-inflammatory effect of curcumin in peripheral blood Mononuclear cells pretreated with lps

Cytokines IL-6, IL-8 and MCP-1 play an important role in a number of inflammatory diseases. Curcumin is known to have strong antioxidant, anti-inflammatory and anti-tumor effects. Observations in patients with severe COVID-19 show an immune response called 'cytokine storm' characterized by fatal hypercytokinemia associated with multiple organ failure. In this aspect, studies focusing on the effect of inhibitors of natural origin on inflammatory response are of interest The aim of the present study was to analyze the effect of curcumin on the secretory levels of pro-inflammatory cytokines from peripheral blood mononuclear cells pre-treated with lipopolysaccharide to induce inflammation. For this purpose, the protein levels of cytokines IL-6, IL-8 and MCP-1 were measured in supernatant using specific kits based on the ELISA method (eBioscience, San Diego, USA). Statistically significant decrease in the secretory levels of cytokines IL-6, IL-8 and MCP-1 was observed and was recorded as early as the second hour after incubation with LPS and curcumin. The levels of MCP-1 secreted by cells treated with both substances were noted to be even lower than those of the control cells at 18 hours, and this dependence was maintained at 24 hours. The decrease in the secretory levels of cytokines IL-6, IL-8 and MCP-1 due to the action of curcumin on peripheral blood mononuclear cells pre-treated with LPS indicates that curcumin inhibits the synthesis and secretion of the studied inflammatory cytokines. © 2021 Medical Information Center. All rights reserved.