Hypobaric Pressure Effects on Gene Expression, as a Physiological Response of Canola Varieties

Aims: The main purpose of our study was to determine the expression levels of genes, which respond to low pressure in canola ( Brassica napus L.) varieties Californium, Orkan, Jura and Elvis. Study Design: Canola ( Brassica napus L.) varieties (Californium, Jura, Orkan and Elvis) placed within specially designed low pressure cabinets were exposed to low pressure (40 Torr ≅ 53 kPa) for durations of 1, 2 or 3 days. Total RNA was isolated from the plants analyzed for genes, OsNCED, OsABA8, OsZEP and TMAC2 by RT-PCR technique. For control, housekeeping gene β-actin was used. As a result OsABA8ox 1, 2, 3 and OsNCED showed increase in expression levels. Methodology: Canola (Brassica napus L.) varieties Californium, Orkan, Jura and Elvis were provided by the Black Sea Agricultural Research Institute, Samsun ‐ Turkey. Seeds were sown and placed in the plant growth chamber. The 14 days old plants were exposed to low pressure in the low pressure cabinet. RT-PCR reactions were performed in onetube reaction according to manufacturer’s protocol (Access Quick RT-PCR System, Promega, A1701). Results: The PCR products of OsNCED, OsZEP, OsABA , TMAC2 separated by 2%