Decreased kidney uptake of technetium-99m-labelled Fab′ fragments in ovarian carcinoma bearing nude mice using a cleavable chelator

Abstract A new 99m Tc labelling method using a cleavable chelator, RP-1, was developed. In this study Balb/c mice with ovarian carcinoma xenografts received various Fab′ fragments labelled with 99m Tc either directly or via RP-1. Kidney uptake was significantly lower for the RP-1 linked conjugates. Tumour uptake showed no significant differences between RP-1 conjugates and directly labelled preparations. In conclusion, with the use of the cleavable linker RP-1, kidney uptake can be reduced significantly resulting in a lower radiation dose to the kidneys.