Interaction of IGF2 and PTEN in (Malignant) Breast Tissues

Results: In-Silico study indicated the absence of hormone responsive elements in the promoters of the selected genes. Methylation results indicated significant loss of methylation in IGF2 exon 9 CpG cluster and significant gain of PTEN promoter methylation in tumors. Immunohistochemistry revealed enhanced cytoplasmic expression of IGF2 protein (p< 0.0001) and decreased nuclear localization of PTEN protein (p=0.0069) in the breast tumors. RT-PCR results indicated an increased IGF2 (p=0.024) and decreased PTEN transcripts (p<0.0001) in the tumors.