Preliminary studies of cell culture strategies for bioprocess development based on HEK293 cells

Background The use of human embryonic kidney cells (HEK293) for recombinant protein or virus production has gained relevance along the last years. They are specially recommended for transient gene expression and adenovirus or adeno-associated virus generation [1,2]. To achieve high volumetric productivities towards bioprocess optimization, the concentration of biocatalizer (i.e. animal cells) must be enhanced. The limits for cell growth are mainly related to the accumulation of metabolic by-products, or the depletion of nutrients [3]; therefore, cell cultures strategies must be developed. In this work, we have explored Punctual Feeding and Media Replacement cell culture strategies to over perform the limit on Xvmax encountered on batch culture mode. Finally, we scaled up cell culture in order to control other parameters (i.e. pO2) that could be limiting cell growth.