慢性乙型肝炎患者HBVDNA载量与PMBC分泌IFN-γ、IL-6水平相关性研究

Objective To investigate the different HBVDNA load in chronic hepatitis B (CHB) patients treated with lamivudine before and after peripheral blood mononuclear cells (PMBC) secretion of IFN-γ, IL-6 level changes and its clinical significance. Methods Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect the quantity of HBV load and enzyme-liked immunosorbent assay (ELISA) was used to determine the levels of INFγ and IL-6 secreted by phytohemagglutinin (PHA) stimulated PBMC. Results IFN-γ level was lower but IL-6 level was higher than that of the control group (P＜0.01) before lamivudine treatment. After lamivudine treatment, IFN-γ levels were significantly increased (P＜0.01 and P＜0.05 respectively), but IL-6 levels were significantly decreased (P＜0.01 and P＜0.05 respectively) in patients from HBVDNA high and middle level groups. However, in patients from HBVDNA middle and low level group, IFN-γ level was lower but IL-6 level was higher than that of control group (P＜0.01). There was a negative correlation between IFN-γ level and the HBVDNA level (r=0.89, P＜0.00 1), but IL-6 level positively correlated with HBVDNA level (r=0.92, P＜0.001) and ALT level (r=0.74, P＜0.001). Conclusion IFN-γ and IL-6 levels secreted by PBMC in CHB patients can be used for the judgment of therapeutic effects during lamivudine treatment.