[The use of Vero (B) cell line for medico-biological preparations].
1996
A variant of Vero cell line has been obtained, adapted to the Russian Eagle's medium with 8% fetal calf serum. The variant is characterized by intensive cell proliferation, possesses a different content of chromosomes, contains no oncogens and contaminants, specifically, Mycoplasma. The strain was characterized and certified in accordance with the WHO requirements. Inoculation (110 ampules) and working (100 ampules) stocks at the levels of the 170th and 178th passages were placed for storage in liquid nitrogen. Vero (B) cell line is sensitive to herpes simplex virus types 1 and 2, CMV, hepatitis A virus, recombinant variolovaccinia strain expressing HbS antigen, etc. The line retains its biological properties from passage 178 to passage 200 and is recommended for control and preparation of various biomedical agents, including antiviral vaccines.
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