Plasma Polymerization of 1,2-Diaminocyclohexane for Covalent Bonding of Bone Morphogenic Protein-2 on Titanium Surface.

Among the bone morphogenic protein (BMP) family members, BMP-2 is a potent osteoinductive factor that plays key roles during bone formation. In this study, plasma polymerization of 1,2-diaminocyclohexane (DACH) was performed to immobilize the BMP-2 on Ti surface. The plasma polymerization of DACH was carried out at a discharge power of 60 W and 100 W under a pressure of 10 mTorr for 90 sec. The BMP-2 was successfully immobilized on the DACH plasma treated Ti surface. The BMP-2 immobilized Ti surface showed the excellent cell differentiation. The results indicate that the DACH plasma polymerized Ti surface has a potential for immobilization of biomolecules in bone tissue engineering.