[Effects of soothing liver and invigorating spleen recipes on SREBP-1c, SCD-1 mRNA and proteins expression in hepatocytes of NAFLD rats].

Objective:To observe the effects of soothing liver and invigorating spleen recipes on the expression levels of SREBP-1c,SCD-1 mRNA and proteins in hepatocytes of NAFLD rats,and to explore its possible mechanisms of prevention and treatment of NAFLD.Methods:75 SD male rats were randomly divided into 5 groups:normal group,model group,soothing liver group( administrated with 9.6 g/kg),invigorating spleen group( administrated with 30 g/kg) and integrated group( administrated with 39.6 g/kg).The rats of NASH model were induced by feeding a high-fat diet.After treatment for 8 weeks,9 rats were randomly taken to detect liver function,TC,TG and pathological changes in liver tissue.The other 6 rats of each group were taken respectively and collagenase( Type Ⅳ) was perfused to digest liver tissue with the circulation in vitro to separate hepatocytes.Real-time Q-PCR and Western Blot were used to detect the expression levels of SREBP-1c,SCD-1 mRNA and proteins.Results:Compared with the model group,the different decrease levels of SREBP-1c,SCD-1 genes and proteins were found in all drug therapy groups( P 0.05 or P 0.01),as well different degrees that liver lipid and pathological changes became better,especially that of in soothing liver group.Comparison between the all drug therapy groups,the hepatocytes expression levels of SREBP-1c and SCD-1 mRNA in soothing liver group were lower than that of in invigorating spleen group( P 0.05),but expression levels of the proteins had no statistical significances.Conclusion:Soothing liver and invigorating spleen recipes prevent and treat NAFLD,its mechanism may be related to inhibiting the activation of SREBP-1c/SCD-1 signal pathway in hepatocytes to down-regulate TC and TG synthesis and reduce hepatic lipid deposition.SREBP-1c,SCD-1 mRNA and proteins may be the effective targets.